书书书G21 G22 G23 G24 G25 G26 G27 G28βG29 G2A G2B G2C２５－３５G2D G2E G2F G30 G31 G32 G33 G34G35 G36 G37 G38 G39 G3A G3BG21 G22，G23 G24 G25，G26 G27 G28，G29 G2A G25，G26 G2B，G2C G2D，G2E G2F G30（G31 G32 G33 G34 G35 G36 G37 G34 G38 G39 G3A，G31 G32 G3B G3C１２１００１）G3C G3D：G3E G37 G21 G22 G23 G24 G25 G26（Ｐｉｏ）G27 G28 G29 G2AβG2B G2C G2D G2E２５－３５（Ａβ２５－３５）G2F G30 G31 G32 G33 G34 G35 G36 G37 G38 G39 G3A G3BG3C G3D G3E G3F G40 G41。G3F G40 G31 G32７ｄG42 G43 G44 G45 G44 G46 G33 G47 G35 G36 G37，G48 G49 G4AＰｉｏ０．０１，０．１，１G4B１０μｍｏｌ·Ｌ－１G40 G41１ｈ，G4C G4D G49 G4AＡβ２５－３５２０μｍｏｌ·Ｌ－１G40 G41２４ｈ；G4E G48 G49 G4AＧＷ９６６２１０μｍｏｌ·Ｌ－１G40 G41３０ｍｉｎG4D，G49 G4AＰｉｏ１μｍｏｌ·Ｌ－１G40 G41１ｈ，G4C G4D G49 G4AＡβ２５－３５G40 G41２４ｈ。ＭＴＴG4F G50 G51 G52 G53 G54 G55 G56；Ｈｏｅｃｈｓｔ３３２５８G57 G58 G59 G21 G22 G52 G53 G5A G5B G42 G5C G5D G5EG5F G60 G61 G52 G53 G5A G5B G56；ＷｅｓｔｅｒｎG62 G63 G4F G64 G50 G65 G66 G67 G68 G69 G6A G6B G55 G42 G2B G2C G6B G6C G42 G6B G6C４（ＭＫＫ４）G6D G6E、G65 G66 G67ｃＪｕｎG6F G70 G71 G6B G6C１（ＪＮＫ１）G6D G6E G4BＢｃｌ２G2B G2C G72 G73 G6D G6E。G41 G42 ＭＴＴG74 G75 G76 G77，Ａβ２５－３５G78 G79 G7A G7B G31 G32 G35 G36 G37 G54 G55G56 G7C G76 G7D G7E（６６．８±１．２）％，Ｐｉｏ０．１，１G4B１０μｍｏｌ·Ｌ－１G78 G7C G76 G27 G7FＡβ２５－３５G80 G81 G42 G31 G32 G35 G36 G37 G54 G55 G56 G82 G7D，G83 G84 G85（８１．２±２．９）％、（８７．９±２．２）％G4B（９８．１±１．９）％，G86 G87 G7C G76 G88 G89 G8A G8B G8C；ＧＷ９６６２G8D G8E G83 G8F G7FＰｉｏG42G90 G91 G92 G93 G40 G41。Ｈｏｅｃｈｓｔ３３２５８G57 G58 G59 G74 G75 G76 G77，G94 G95 G31 G32 G35 G36 G37 G52 G53 G57 G96 G97 G58 G59 G98 G99，G9A G9B G7E G9C G9D G9E G52 G53G9F GA0 G57 GA1 GA2，G5A G5B G56 G85（１１．８±１．１）％。Ａβ２５－３５G40 G41２４ｈG4D，G35 G36 G37 G9F GA0 G57 GA1 GA2 G4E G57 GA3 G69 G42 GA4 GA5 G7C G76 GA6 GA7，G5A G5B G56 GA6 G49 G7E（６４．６±２．３）％，Ｐｉｏ０．１，１G4B１０μｍｏｌ·Ｌ－１G78 G7C G76 G92 G93Ａβ２５－３５GA8 GA9 G42 G31 G32 G35 G36 G37 G5A G5B G56 GA6G49，G5A G5B G56 G83 G84 G85（５８．３±１．２）％、（５２．６±１．３）％G4B（４１．５±１．５）％。ＷｅｓｔｅｒｎG62 G63 G74 G75 G76 G77，GAA G94 G95 G27 GAB GACGAD GAE，Ａβ２５－３５G78 G79 G35 G36 G37Ｂｃｌ２G2B G2C G72 G73 G6D G6E G7C G76 G7D GAF，G65 G66 G67ＭＫＫ４G72 G73 G61 G65 G66 G67ＪＮＫ１G72 G73 G6D G6E G7C G76 GA6G49，ＰｉｏG8D GB0 GB1Ｂｃｌ２G42 G72 G73 G6D G6E，G27 G7FＡβ２５－３５G80 G81 G42 G65 G66 G67ＭＫＫ４G4B G65 G66 G67ＪＮＫ１G72 G73 G6D G6E GA6 G49。G41 G43ＰｉｏG8D GB2 G7C G76 G92 G93Ａβ２５－３５GA8 GA9 G7A G7B G31 G32 G35 G36 G37 G42 G38 G39 G40 G41，G90 G91 G40 G41 G78 G8D GAA G6B G55 GB3 GB4 G67 GB5 G6C G7A GA6 GB6 GB5 G6B G55GB7 G7Aγ、GB0 GB1Ｂｃｌ２G2B G2C G72 G73 G4B G92 G93ＭＫＫ４／ＪＮＫGB8 GB9 GBA G81 GBB GBC G3D GBD。G44 G45 G46：G23 G24 G25 G26；G28 G29 G2AβG2B G2C；GBE GBF GC0 GC1 GC2 GC3；G52 G53 G5A G5B；ｃＪｕｎG6F G70 G71 G6B G6CG47 G48 G49 G4A G4B：Ｒ９６６ G4C G4D G4E G4F G50：Ａ G4C G51 G52 G4B：１０００３００２（２０１１）０１００３４０６ ＤＯＩ：１０．３８６７／ｊ．ｉｓｓｎ．１０００３００２．２０１１．０１．００７ G3D G3E G3F G2A G40 G41（Ａｌｚｈｅｉｍｅｒｄｉｓｅａｓｅ，ＡＤ）G42 G43 G44G45 G46 G47 G48 G49 G4A G4B G4C G4D G4E G41，G4F G50 G51 G52 G53 G47 G48 G41 G37 G34G54 G55 G42 G56 G2A G57 G58 G59 G5A G5B G5C G47 G48 G5D G5E G5F G60 G61 G62 G59 G63 G64G47 G48 G65 G4B G4C G4D G66 G67 G53 G68 G69 G6A、G47 G48 G5D G5E G6B G61 G62 G47 G48G65 G6C G6D G6E G6F（ｎｅｕｒｏｆｉｂｒｉｌｌａｒｙｔａｎｇｌｅｓ，ＮＦＴ）G58 G70 G51 G53G71 G5C G72［１］。G4F G73 G41 G74 G75 G76 G77 G78 G79，G7A G7B G66 G59 G63 G7C G67G53 G7D G7E G7F G80 G81 G70，G82 G83 G84 G85 G86 G53 G87 G88（ｂｅｔａＡｍｙｌｏｉｄ，Ａβ）G42ＡＤG73 G41 G53 G45 G89 G8A G8B。G68 G69 G6A G53 G8C G8D G8E G8F G90βG82 G83 G84 G85 G86，ＡβG42 G82 G83 G84 G85 G86 G91 G92（ａｍｙｌｏｉｄｐｒｏｔｅｉｎｐｒｅｃｕｒｓｏｒ，ＡＰＰ）G48 G93 G77 G94 G53 G95（α、β、γG8F G96G95）G56 G77 G94 G97 G98 G99 G9A G9B G8E，G9C３９～４３（Ａβ１－４０）G9D G53 G54 G55 G3E：G31 G32 G9E G38 G9F GA0 GA1 GA2 GA3 GA4 GA5 GA6（ＬＴ２０１００６４）；G31 G32 G9E GA7 GA8 GA9 G34 GAA G21 G7F GAB GA5 GA6（２００４２１７１） G3A G56 G57 G58：G21 G22（１９６０－），GAC，G38 GAD，GAE GAF，G8C G8D GB0GB1 G47 G48 G4B G4C G4D G4E G41 G53 G73 G41 G74 G75 G64 G36 GB2 GB3 GB4 G39 GB5。 G59 G5A G3A G56：G21 G22，Ｅｍａｉｌ：ｊｙｊｉｎｙｉｎｇ＠ｙａｈｏｏ．ｃｏｍ．ｃｎ，Ｔｅｌ：（０４１６）４６７３４０９４２～４３（Ａβ１－４２，４３）GB6 GB7 GAA GB8 GB9 G8E，G4F GBA G89 GBB GBC G90Ａβ２５－３５［２］。G7B G66 G39 GB5 G81 G70 G5D G5E G6BＡβGBD GBE GBF G5B GC0 G47G48 G65 GC1 GC2［３－４］。GC3 GC4 GC5 GC6（ｐｉｏｇｌｉｔａｚｏｎｅ，Ｐｉｏ）GC7 GC8 GC9 GCA GCB GCC GC6（ｔｈｉａｚｏｌｉｄｉｎｅｄｉｏｎｅｄｅｒｉｖａｔｉｖｅｓ，ＴＺＤ）GCD G36 GB2，G42ＰＰＡＲγGCE G92 G66 GCF G53 GD0 GD1 GD2［５］。ＰＰＡＲγGCE G92 GD0 GD1 GD2G56 GD3 GD4 G5D G5E G8F G72、GD5 GD6 G41、GD7 GD8 GD9 GDA GDB、GD1 GDC GDD G84 GDEG72 GDF G8E、GDB GE0 GE1、GDB GE2 GE3 GE4 GDB GE5 GE6 GE7 GE8 G45 GE9 GEA GEB G8D GECGED。G7B G66 G39 GB5 G81 G70，ＰｉｏGD0 GD1ＰＰＡＲγGCE G92 GEE G75βG8FG96 G95ＢＡＣＥ１GAA GEF GF0 GD1 GF1 G53 GF2 G4D，GF3 GF4ＡβG53 GF5GE1［６－７］。GF6 GF7 G7D G7E G3A G39 GB5 G81 G70，ＰｉｏGF8 GF9 GDB G5A G3A G6B GFAGFBＡβGFC GEA G53 G34 GFD GFE GFF G21 G22［８］G58 G47 G48 G5D G5E GC1 GC2［９］，G23 G44 G24 G25 GEC GED G64 GEE G75Ｐ３８G26 G27 G28 GF2 G72 G85 G86 GD0 G95（Ｐ３８ｍｉｔｏｇｅｎａｃｔｉｖａｔｅｄｐｒｏｔｅｉｎｋｉｎａｓｅ，Ｐ３８ＭＡＰＫ）G29G2A［１０］G58ｃＪｕｎGB7 GAA G2B GD0 G95（ｃＪｕｎＮｔｅｒｍｉｎａｌｋｉｎａｓｅｓ，ＪＮＫ）G29 G2A G66 G67［１１］。G7A G67 GC8ＰｉｏGF9ＡβGFC GEA G53 G92 G2CG2D G2E G5B G5C G47 G48 G65 G2F G30 G42 G31 G66 G24 G25 GEC GED GA6 G91 G76 G32 G33 G34·４３·GC4 GC5 GC6 GC7 G5E GAA GC8 GC7 G5E GC9 GCA２０１１GCB２GCC GCD２５GCE GCD１GCF ＣｈｉｎＪＰｈａｒｍａｃｏｌＴｏｘｉｃｏｌ，Ｖｏｌ２５，Ｎｏ１，Ｆｅｂ２０１１G35，G36 G39 GB5 GED G2D G2E G53 G59 G37 G38 G37 G5B G5C G47 G48 G65 G39 G3AＰｉｏGF9Ａβ２５－３５GFC GEA G53 G47 G48 G2F G30 G42 G31 G66 G24 G25 GEC GED。１ G5B G5C G5D G3F G40１．１ G5E G5F G60 G61 G62ＰｉｏG9C G3B G36ＴａｋｅｄａG3C G3D G3E G3F，G40 G41：０６０９０１，G42G43＞９９％，GED GCC G44 G45 G46（ＤＭＳＯ）G47 G48。Ａβ２５－３５G49 GA7ＳｉｇｍａG3C G3D，GEDＤＭＥＭ（Ｄｕｌｂｅｃｃｏ′ｓＭｏｄｉｆｉｅｄＥａｇｌｅＭｅｄｉｕｍ）G47 G48，３７℃G4A G9F４８ｈ，G4B G4F G8E G90 G4C G4D G4E G4F；ＰＰＡＲγG54 G50 G4D G51 G52 GD2ＧＷ９６６２（G53 G41ＣＡＹ７０７８５Ｍ００１）G49 GA7 G54 G55 G56 G57 G66 G58 G3C G3D；ＤＭＳＯ，ＤＭＥＭG2D G2EGAA，Ｆ１２G2D G2E GAA，Ｈｏｅｃｈｓｔ３３２５８，GD7 G85 G86 G95（１∶２５０），ＬG59 G4C G5A GB7 GB8 GE9 G49 GA7ＳｉｇｍａG3C G3D；ＭＴＴG5B GD2 G49 GA7 G5CG5D G5E GE1 GB2 G5F G60 G39 GB5 G61；GA2 GE1 G62 G63 G64 G78、４G65 G66 GAA G67 G68G66 G69 GB8〔４（２ｈｙｄｒｏｘｙｅｔｈｙｌ）１ｐｉｐｅｒａｚｉｎｅｅｔｈａｎｅｓｕｌｆｏｎｉｃａｃｉｄ，ＨＥＰＥＳ〕G58 G57 G64 G78 G49 GA7 G6A G6B G6C G6D G6E GBE GA9 G5FG66 G58 G3C G3D；G6F GB8 G72 G26 G27 G28 GD0 GF2 G53 G85 G86 GD0 G95 G53 GD0 G95４（ｍｉｔｏｇｅｎａｃｔｉｖａｔｅｄｐｒｏｔｅｉｎｋｉｎａｓｅｋｉｎａｓｅ４，ＭＫＫ４），G6F GB8 G72ＪＮＫ１（Ｔｈｒ１８３／Ｔｙｒ１８５），ＭＫＫ４，ＪＮＫ１GDB G92GE9 G49 GA7ＣｅｌｌＳｉｇｎａｌｉｎｇG3C G3D；G70 GDB G47 G48 G65 G54 G50 G4D G71 G72G72 G95（ｎｅｕｒｏｎｓｐｅｃｉｆｉｃｅｎｏｌａｓｅ，ＮＳＥ）G59 G73 G74 GDB G92，G37GDB G47 G48 G26 G85 G86（ｎｅｕｒｏｆｉｌａｍｅｎｔ，ＮＦ２００）G75 G73 G74 GDB G92，G76 G77 GDB G70 G58 G76 G77 GDB G37 GCC GDB GE9 G49 GA7ＳａｎｔａＣｒｕｚG3C G3D；βG78 GD1 G85 G86 GDB G92 G58ＮＢＴ／ＢＣＩＰG51 G79 G7A G49 GA7ＢｅｙｏｔｉｍｅG5B GD2 G3C G3D。１．２ G2E G2F G30 G31 G32 G33 G34 G63 G64 G65 G49 G66G7B G61 GE1２４ｈG7C G6B G53ＳｐｒａｇｕｅＤａｗｌｅｙG59 G37 G38 G37〔GD1 GB2 G7D GC4 G7E G41：ＳＣＸＫ（G31）２００３０００７，G31 G32 G33 G34 G35GD1 GB2 G7D G7E G45 G89 G3E G3F〕，７５％G66 G72 G7F G80 G81 G82，G83 G84 G85 G86G87 G7B G61 G59 G5A G5B GC0，G88 GC8 G2D G2E GAA G45，G89 G8A G64 G8B G58 G8C G5AG8D，G99 G8E１ｍｍ３G8E G8F G53 G90 G91。G92 G93０．１２５％GD7 G85 G86G95３７℃G81 G72１０ｍｉｎ。G94 G5D G5E G8F G95 G96，G92 G93 G97１０％G62G63 G64 G78 G58１０％G57 G64 G78 G53ＤＭＥＭ／Ｆ１２G2D G2E GAA G98 G99 G81G72。２００GA6 G9A G9B G93 G9C，１０００×ｇG9D G89１０ｍｉｎ，GED G97１０％G62 G63 G64 G78 G58１０％G57 G64 G78 G53ＤＭＥＭ／Ｆ１２G2D G2E GAAG75 G8E G5D G5E G9E G7A，G9F GA0 G5D G5E GA1 G43 G90１×１０８～１×１０９Ｌ－１，GA2 G44 G56 GA3 G66ＬG59 G4C G5A GB7 GB8２４GA4 G9D６GA4 G2D G2E GA5 G45，G88 GC8３７℃，５％ＣＯ２G2D G2E GA6 G45 G2D G2E。G2D G2E４８ｈG96 G92G93 G97 G3D GD5 G5E GA7（G98 GA8 G43 G90５ｍｇ·Ｌ－１）G53 G2D G2E GAA，GEEG75 GA9 G47 G48 G5D G5E GE0 GAA。G7C G96 GAB３ｄGAC G7A１GAD，G2D G2E７ｄG96 GED GC8 G7D G7E［１０］。GE8 GED GDBＮＳＥG59 G73 G74 GDB G92 G58ＮＦ２００GDB G92 GAE GAF GB0 GB1 GB2 G79 GB3 G4C G47 G48 G65 G42 G43 GB4 GB5，G6F GB6 G81 G70G47 G48 G65 G42 G43 G56９０％G7C GB7。G5D G5E G8F G90 GB8 GB9 GF9 GBA GB9（G92 G93 GBB G63 G2D G2E GAA），GBC GBDGB9（G92 G93Ａβ２５－３５２０μｍｏｌ·Ｌ－１GEC GED２４ｈ），ＰｉｏGB9（G8FGBE G92 G93Ｐｉｏ０．０１，０．１，１G58１０μｍｏｌ·Ｌ－１GEC GED１ｈ，GA8G96 G92 G93Ａβ２５－３５GEC GED２４ｈ），Ｐｉｏ＋ＧＷ９６６２GB9（GBF G92G93ＧＷ９６６２１０μｍｏｌ·Ｌ－１GEC GED３０ｍｉｎG96 G92 G93Ｐｉｏ１μｍｏｌ·Ｌ－１GEC GED１ｈ，GA8 G96 GC0 G92 G93Ａβ２５－３５GEC GED２４ｈ）。１．３ ＭＴＴG40 G67 G68 G69 G6A G6B G6C G6DGC1 GA2 G44 G56９６GA4 G2D G2E GA5 G53 G47 G48 G65 GC2 G7C GB7 G8F GB9 GC3G37 G96，G92 G93ＭＴＴ（G98 GA8 G43０．５ｇ·Ｌ－１）G2D G2E４ｈ，GC4 GC5GB7 G78 G7A，GAB GA4 G92 G93１５０μｌＤＭＳＯ，G94 GC6 GC7 G47 G48 G96，G56５７０ｎｍG53 GC8 GB1 GC9 G87 GCA GCB GCC GA4 G53 GC8 GB1 G43（ａｂｏｓｒｂａｎｃｅ，Ａ），GCD GCE GCF G3C GD0 GD1 GD2 G5D G5E GD3 GF2 GD4。G5D G5E GD3 GF2 GD4（％）＝（ＡG7D G7E GB9－ＡGD5 G86 GB9）／（ＡGB8 GB9 GF9 GBA GB9－ＡGD5 G86 GB9）×１００％。１．４ Ｈｏｅｃｈｓｔ３３２５８G6E G6F G70 G71 G67 G32 G33 G34 G72 G73GB8 GB9 GF9 GBA GB9、GBC GBD GB9 G58ＰｉｏGB9 G5D G5E GD6 GC5 G2D G2E GAA，GED GD7 G6F GB8 GD8 GD9 GDA G7A（ｐｈｏｓｐｈｏｒａｔｅｂｕｆｆｅｒｅｄｓａｌｉｎｅ，ＰＢＳ）GDB２GAD，４％G53 G59 G4C G44 GDC GDD GB5２０ｍｉｎ，GC4 GDD GB5G7A，GDE GDF GE0 GDA GDB２GAD，Ｈｏｅｃｈｓｔ３３２５８５ｍｇ·Ｌ－１GE1 GB1GB2 G79１０ｍｉｎ，GED GDE GDF GE0 GDA GDB２GAD，G3A GE2 GE3 GE4 G96 GC8 GB0 GB1G51 GE5 GE6（Ｏｌｙｍｐｕｓ）G87 G39 G3A GE7 GBA。GAB GB9 G7B４G23 GE8 GE9GBB，GAB G23 GBB GEA G74 GEB G7B４GB6 GEC GED GEE GEF，GD1 GD2 GC1 GC2 G5D G5E GF0GAB GB6 GEE GEF GF1 G5D G5E GF2 G53 GF3 G8F GD4，GF4 G61４GB6 GEE GEF GF5 GE9 GF3G8F GD4，GEC G90 GF6 G84 G36 G53 GC1 GC2 GF3 G8F GD4。１．５ ＷｅｓｔｅｒｎG74 G75 G40 G71 G67Ｂｃｌ２，G76 G77 G78ＭＫＫ４G65ＪＮＫ１G29 G2A G79 G7AGB8 GB9 GF9 GBA GB9、GBC GBD GB9 G58ＰｉｏGB9 G5D G5E GED GD7 G53ＰＢＳGDA GDB，GF7 GF8 GF9 G93 GFA GD7 G53 G27 G48 GD9 GDA G7A G45〔１％Ｔｒｉｔｏｎ，０．１％ＳＤＳ，０．５％GC5 GFB GFC GB8，ＥＤＴＡ１ｍｍｏｌ·Ｌ－１，Ｔｒｉｓ（ｐＨ７．４）２０ｍｍｏｌ·Ｌ－１，１ＮａＣｌ５０ｍｍｏｌ·Ｌ－１，ＮａＦ１０ｍｍｏｌ·Ｌ－１，GFD G44 GAA G69 GFE GFF G72 GB2（ＰＭＳＦ）０．１ｍｍｏｌ·Ｌ－１〕，４℃G21 G22 G83 G23 G96，１２０００×ｇG9D G89３０ｍｉｎ，G7B GB7 G78，GEDＬｏｗｒｙGBB G24 GCB GB5 G85 G86 G5C G97 G63，G7C G63 G64G78 G86 G85 G86（ＢＳＡ）G90 G25 G26 G27，GC1 GCC GB9 G85 G86 GA8 G43 G9F G8E G43GBF。GED１０％～１２％G53ＳＤＳG4C G28 G29 GFE G2A G2B G71 G2C G2D（ＳＤＳＰＡＧＥ）G8F G9D G85 G86 G5C，GAB GB6 G2D G35 G85 G86 GB7 G84 G63 G90２０μｇ。G90 G2E G26 G2F G30 G52 GA6 G53 G85 G86 G31 G53 G97 G88，G43 GB6 G2D G35G92ＳｅｅＢｌｕｅＰｌｕｓ２GFA GB2 G85 G86 G25 GFE GB2，G2C G2D G96 GC1ＰＡＧＥG2B G71 G45 G53 G85 G86 G5C G2C G6E GD6 G32 G33 GB8 G6C G6D GD9 G8D GB7，G7B G61 G96GC1 G8D GF9 G93３％ＢＳＡG51 G52 GD9 GDA G7A G45，G34 G35６０ｍｉｎ，GC0 GEDＴＢＳ〔Ｔｒｉｓ（ｐＨ８．０）１０ｍｍｏｌ·Ｌ－１，ＮａＣｌ１５０ｍｍｏｌ·Ｌ－１）GDB G8D３GAD，GAB GAD１０ｍｉｎ。GC1 G8D GF9 G93 G43 GDB G45（GDB G92１∶５００G71 G36），４℃G93 G37。ＴＴＢＳGDA GDB G96，GC1 G8D GF9 G93 GCC GDB（GCC GDB GE9１∶５００G71 G36）G45，G3A GE2 G4A G9F１～２ｈ，GA8 G96 GEDＴＴＢＳGDB G8D３GAD，GAB GAD１０ｍｉｎ，G88 G33 GAA G38 G39 GCA G3A／５G3B４G39３G3C G3D G6F GB8 GD8（ＮＢＴ／ＢＣＩＰ）G51 G79 G7A G45 G51 G79，G3EG32 G61 G62，G98 G99 GE7 GE8。GAB GB6 GDB G92 GCB GB5 G3F G40 GC1 G64βG78 GD1·５３·GC4 GC5 GC6 GC7 G5E GAA GC8 GC7 G5E GC9 GCA２０１１GCB２GCC GCD２５GCE GCD１GCF ＣｈｉｎＪＰｈａｒｍａｃｏｌＴｏｘｉｃｏｌ，Ｖｏｌ２５，Ｎｏ１，Ｆｅｂ２０１１G85 G86 G5C G7C GE8 G53 G8D GEDＴＢＳGDB３GAD G96，GC1 G8D GF9 G93βG78 GD1G85 G86 GDB G92 G45，４℃G93 G37，GB3 G4C G41 G42，ＴＴＢＳGDA GDB G96，GC1 G8DGF9 G93 GCC GDB G45，G3A GE2 G4A G9F１ｈ。GC0 GEDＴＴＢＳGDB G8D３GADG96，GF9 G93ＮＢＴ／ＢＣＩＰG51 G79 G7A G45 GE1 GB1 G51 G79，G3E G32 G51 G79，G98 G99 GE7 GE8，G7C G24 G7E G85 G86 GB7 G84 G63 G53 G43 GBF G4D。GC1 G85 G86 G43G44 G51 G45 G46 G47 G48，G49 GED G2B G71 GA7 GD1 G8F G4A G8E G4B G8C G86ＣｈｅｍＩｍａｇｅ５５００GF9 G85 G86 G31 GB3 G4C G8F G4A，G7C GA6 G25 G85 G86 G64βG78GD1 G85 G86 G53 G88 G8F GC8 GB1 G43（ｉｎｔｅｇｒａｔｅｄａｂｓｏｒｂａｎｃｅ，ＩＡ）GC9G53 G4C GC9 G81 G4D G85 G86 G53 G7C GF9 G81 G4E G63。１．７ G7B G7C G7D G49 G7EG7D G7E G6F GB6 GF2 GCF GED珋ｘ±ｓG81 G4D，G4F GEDｏｎｅｗａｙＡＮＯＶＡG58ＬＳＤ′ｓｐｏｓｔｈｏｃｔｅｓｔGB3 G4C G4A GD1 G34 G8F G4A。２ G41 G42２．１ G21 G22 G23 G24 G25Ａβ２５－３５G7F G80 G37 G32 G33 G34 G6B G6C G6D G37G81 G82G81１G6F GB6 G51 G4D，Ａβ２５－３５GEC GED２４ｈGBD G4B G59 G37 G59G5A G5B G5C G47 G48 G65 GD3 GF2 GD4 G70 G51 G50 G51；Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GBD G70 G51 GF9 GDBＡβ２５－３５G52 GBE G53 G47 G48 G65 GD3 GF2 GD4G87 G50，GCD G53 G70 G51 GA8 G43 G54 G5A G4D（ｒ＝０．９８３，Ｐ＜０．０１）。Ｐｉｏ０．０１μｍｏｌ·Ｌ－１GF9Ａβ２５－３５G52 GBE G53 G47 G48 G65 GD3 GF2 GD4G87 G50 G55 G66 G70 G51 GF9 GDB GEC GED。ＰＰＡＲγGCE G92 G53 G54 G50 G4D G51G52 GD2ＧＷ９６６２１０μｍｏｌ·Ｌ－１GF8 G56 G8F GF9 GDBＰｉｏGF9Ａβ２５－３５GFC GEA G53 G47 G48 G65 GD3 GF2 GD4 G87 G50 G53 GEE G75 GEC GED。G75G57 GEDＧＷ９６６２１０μｍｏｌ·Ｌ－１GF9 G47 G48 G65 GD3 GF2 GD4 G55 G66G45 G58。G79１ G21 G22 G23 G24（Ｐｉｏ）G25 G26 G27 G28βG29 G2A G2B G2C２５－３５（Ａβ２５－３５）G7F G80 G37 G32 G33 G34 G6B G6C G6D G37 G81 G82Ｔａｂ．１ Ｅｆｆｅｃｔｓｏｆｐｉｏｇｌｉｔａｚｏｎｅ（Ｐｉｏ）ｏｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｕｒｖｉｖａｌｗｉｔｈａｎｙｌｏｉｄβｐｒｏｔｅｉｎｆｒａｇｅｍｅｎｔ２５－３５（Ａβ２５－３５）ｉｎｊｕｒｙGB9 GBE G5D G5E GD3 GF2 GD4／％GB8 GB9 GF9 GBA１００．０±１．９Ａβ２５－３５GBC GBD６６．８±１．２Ａβ２５－３５＋Ｐｉｏ０．０１７１．６±３．８ ０．１８１．２±２．９＃＃ １８７．９±２．２＃＃ １０９８．１±１．９＃＃Ａβ２５－３５＋Ｐｉｏ１＋ＧＷ９６６２１０７３．５±３．４△△ＧＷ９６６２１０９６．９±３．１G47 G48 G65 GBF G64 G36 GB2 GEC GED１ｈG96 G92 G93Ａβ２５－３５２０μｍｏｌ·Ｌ－１GEC GED２４ｈ；ＧＷ９６６２１０μｍｏｌ·Ｌ－１GBF G92，GEC GED３０ｍｉｎG96 G92 G93Ｐｉｏ１μｍｏｌ·Ｌ－１GEC GED１ｈ。G5D G5E GD3GF2 GD4（％）＝（ＡG7D G7E GB9－ＡGD5 G86 GB9）／（ＡGB8 GB9 GF9 GBA GB9－ＡGD5 G86 GB9）×１００％．珋ｘ±ｓ，ｎ＝１５．Ｐ＜０．０１，G64 GB8 GB9 GF9 GBA GB9 G4C G59；＃＃Ｐ＜０．０１，G64Ａβ２５－３５GBC GBD GB9 G4C G59；△△Ｐ＜０．０１，G64Ａβ２５－３５＋Ｐｉｏ１μｍｏｌ·Ｌ－１GB9 G4C G59．２．２ G21 G22 G23 G24 G25Ａβ２５－３５G7F G80 G37 G32 G33 G34 G72 G73 G37 G81 G82Ｈｏｅｃｈｓｔ３３２５８GBA GB2 G79 G73 G62，GB8 GB9 G2D G2E G47 G48 G65 G5DG5E GBA GB0 GB1 GB2 G79 GE9 G5A，G5B G33 G5C G5D GF4 G63 G5D G5E G61 G62 GBA GDD G5E（G46１Ａ）。Ａβ２５－３５GB9 G5D G5E G61 G62 G70 G51 GC1 GC2 G54 G55 G4D G5F G60：GBA G53 GA8 GB2 G53 G61 G62 GDF G9D G23 G91 G4E GB0 GB1（G46１Ｂ）。G61 GB5 G63 G6FGB6 G51 G4D，GB8 GB9 G47 G48 G65 GC1 GC2 G5D G5E GF3 G8F GD4 G90（１１．８±１．１）％，G92 G93Ａβ２５－３５２０μｍｏｌ·Ｌ－１GEC GED２４ｈ，GC1 GC2 G5DG5E GF3 G8F GD4 G4E G5C（６４．６±２．３）％，G70 G51 GE0 G92（Ｐ＜０．０１）。Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GBD G70 G51 GF9 GDBＡβ２５－３５G52 GBE G53 G47 G48 G65 GC1 GC2，G8F GBE G90（５８．３±１．２）％，（５２．６±１．３）％G58（４１．５±１．５）％（Ｐ＜０．０５）。G48１ ＰｉｏG25Ａβ２５－３５G7F G80 G37 G32 G33 G34 G72 G73 G37 G81 G82（Ｈｏｅｃｈｓｔ３３２５８GB2 G79×４００）．G8F GB9 GC3 G37 G33 G81１．Ａ：GB8 GB9 GF9 GBA GB9；Ｂ：GBC GBDGB9；Ｃ：Ａβ２５－３５＋Ｐｉｏ１μｍｏｌ·Ｌ－１GB9．Ｆｉｇ．１ ＥｆｆｅｃｔｏｆＰｉｏｏｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓａｐｏｐｔｏｓｉｓｉｎｄｕｃｅｄｂｙＡβ２５－３５（Ｈｏｅｃｈｓｔ３３２５８ｓｔａｉｎｉｎｇ×４００）．２．３ G21 G22 G23 G24 G25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32 G33 G34 G85 G72G73 G29 G2AＢｃｌ２G79 G7A G37 G81 G82G81２G58 G46２G6F GB6 G51 G4D，G64 GB8 GB9 GF9 GBA GB9 G7C G4C，Ａβ２５－３５２０μｍｏｌ·Ｌ－１GBD G4B G47 G48 G65Ｂｃｌ２G85 G86 G81 G4E GE0·６３·GC4 GC5 GC6 GC7 G5E GAA GC8 GC7 G5E GC9 GCA２０１１GCB２GCC GCD２５GCE GCD１GCF ＣｈｉｎＪＰｈａｒｍａｃｏｌＴｏｘｉｃｏｌ，Ｖｏｌ２５，Ｎｏ１，Ｆｅｂ２０１１G79２ ＰｉｏG25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32 G33 G34 G85 G72 G73 G29 G2AＢｃｌ２G79 G7A G86 G87 G37 G81 G82Ｔａｂ．２ ＥｆｆｅｃｔｏｆＰｉｏｏｎＡβ２５－３５ｉｎｄｕｃｅｄｄｏｗｎｒｅｇｕｌａｔｉｏｎｏｆＢｃｌ２ｐｒｏｔｅｉｎｌｅｖｅｌｉｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓGB9 GBEＢｃｌ２G85 G86 G81 G4E（ＩＡＢｃｌ２∶ＩＡβG78 GD1 G85 G86）GB8 GB9 GF9 GBA０．９１８±０．１１３Ａβ２５－３５GBC GBD０．５９１±０．０２１Ａβ２５－３５＋Ｐｉｏ０．１０．６７９±０．０３６＃ １０．７４４±０．０２１＃＃ １００．８３４±０．０４０＃＃G8F GB9 GC3 G37 G33 G81１．珋ｘ±ｓ，ｎ＝３．Ｐ＜０．０１，G64 GB8 GB9 GF9 GBA GB9 G4C G59；＃Ｐ＜０．０５，＃＃Ｐ＜０．０１，G64Ａβ２５－３５GBC GBD GB9 G4C G59．G48２ ＷｅｓｔｅｒｎG74 G75 G40 G71 G67ＰｉｏG25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32G33 G34 G85 G72 G73 G29 G2AＢｃｌ２G79 G7A G86 G87 G81 G82．G85 G31１：GB8 GB9GF9 GBA GB9；G85 G31２：Ａβ２５－３５GBC GBD GB9；G85 G31３，４，５：G8F GBE G90Ａβ２５－３５＋Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GB9．Ｆｉｇ．２ ＥｆｆｅｃｔｏｆＰｉｏｏｎＡβ２５－３５ｉｎｄｕｃｅｄｄｏｗｎｒｅｇｕｌａｔｉｏｎｏｆＢｃｌ２ｐｒｏｔｅｉｎｌｅｖｅｌｉｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｂｙＷｅｓｔｅｒｎｂｌｏｔｔｉｎｇ．GF5 G70 G51 G50 G51。Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GF8 G70 G51 GF9GDBＡβ２５－３５GFC GEAＢｃｌ２G85 G86 G81 G4E GE0 GF5 G53 G50 G51，G4FGEC GED G53 G70 G51 G53 GA8 G43 G54 G5A G4D（G81２）（ｒ＝０．８９６，Ｐ＜０．０１）。２．４ G21 G22 G23 G24 G25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32 G33 G34 G76 G77G78ＭＫＫ４G29 G2A G86 G87 G37 G81 G82G81３G58 G46３G6F GB6 G51 G4D，G64 GB8 GB9 GF9 GBA GB9 G7C G4C，G92 G93Ａβ２５－３５２０μｍｏｌ·Ｌ－１GEC GED２４ｈGBD G4B G47 G48 G65 G6F GB8 G72ＭＫＫ４GE0 GF5 G70 G51 GE0 G92。Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GBDG70 G51 GEE G75Ａβ２５－３５GFC GEA G53 G47 G48 G65 G6F GB8 G72ＭＫＫ４GE0 GF5GE0 G92，GCD G53 G43 GB5 GA8 G43 G54 G5A G4D（ｒ＝０．８９３，Ｐ＜０．０１）。G7A GCC GB9 G60ＴＭＫＫ４G85 G86 G81 G4E GE0 GF5 G55 G66 G70 G51 G63 G50。２．５ G21 G22 G23 G24 G25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32 G33 G34 G76 G77G78ＪＮＫ１G29 G2A G86 G87 G37 G81 G82G81４G58 G46４G6F GB6 G51 G4D，G64 GB8 GB9 GF9 GBA GB9 G7C G4C，G92 G93Ａβ２５－３５２０μｍｏｌ·Ｌ－１GEC GED２４ｈGBD G4B G2D G2E G47 G48 G65 G6F GB8G72ＪＮＫ１GE0 GF5 G70 G51 GE0 G92，G7AＴＪＮＫG81 G4E G64 G65 G55 G66 G5FG60。Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GBD G70 G51 GF9 GDBＡβ２５－３５GFC GEA G53 G47 G48 G65 G6F GB8 G72ＪＮＫ１GE0 GF5 G70 G51 GE0 G92，GCD G53 G43GB5 GA8 G43 G54 G5A G4D（ｒ＝０．６９５，Ｐ＜０．０５）（G81４）。G7A GCC GB9G60ＴＪＮＫG81 G4E G55 G66 G70 G51 G63 G50。G79３ ＰｉｏG25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32 G33 G34 G76 G77 G78 G88 G89 G8A G8BG6C G37 G29 G2A G8B G8C G37 G8B G8C４（ｐＭＫＫ４）G29 G2A G86 G87 G37 G81 G82Ｔａｂ．３ ＥｆｆｅｃｔｏｆＰｉｏｏｎｐｈｏｓｐｈｏｒｙｌａｔｅｄｍｉｔｏｇｅｎａｃｔｉｖａｔｅｄｐｒｏｔｅｉｎｋｉｎａｓｅｋｉｎａｓｅ４（ｐＭＫＫ４）ｐｒｏｔｅｉｎｌｅｖｅｌｉｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓGB9 GBEｐＭＫＫ４G85 G86 G81 G4E（ＩＡｐＭＫＫ４∶ＩＡＴＭＫＫ４）GB8 GB9 GF9 GBA０．４４３±０．０１３Ａβ２５－３５GBC GBD０．６８１±０．００５Ａβ２５－３５＋Ｐｉｏ０．１０．５４１±０．０５８ １０．４９１±０．００７＃＃ １００．４０７±０．０５９＃＃G8F GB9 GC3 G37 G33 G81１．珋ｘ±ｓ，ｎ＝３．Ｐ＜０．０１，G64 GB8 GB9 GF9 GBA GB9 G4C G59；＃＃Ｐ＜０．０１，G64Ａβ２５－３５GBC GBD GB9 G4C G59．G48３ ＰｉｏG25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32 G33 G34ｐＭＫＫ４G29 G2A G86 G87 G81G82 G37ＷｅｓｔｅｒｎG74 G75 G41 G42．G85 G31１：GB8 GB9 GF9 GBA GB9；G85 G31２：GBC GBD GB9；G85 G31３～５：G8F GBE G90Ａβ２５－３５＋Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GB9．Ｆｉｇ．３ ＲｅｐｒｅｓｅｎｔａｔｉｖｅＷｅｓｔｅｒｎｂｌｏｔｔｉｎｇｉｎｅｘｐｅｒｉｍｅｎｔｏｆＴａｂ．３．G79４ ＰｉｏG25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32 G33 G34 G76 G77 G78ｃＪｕｎG8D G53G8E G8B G8C１（ｐＪＮＫ１）G29 G2A G86 G87 G37 G81 G82Ｔａｂ．４ ＥｆｆｅｃｔｏｆＰｉｏｏｎＡβ２５－３５ｉｎｄｕｃｅｄｐｈｏｓｐｈｏｒｙｌａｔｅｄｃＪｕｎＮｔｅｒｍｉｎａｌｋｉｎａｓｅｓ１（ｐＪＮＫ１）ｐｒｏｔｅｉｎｌｅｖｅｌｉｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓGB9 GBEｐＪＮＫ１G85 G86 G81 G4E（ＩＡｐＪＮＫ１∶ＩＡＴＪＮＫ）GB8 GB9 GF9 GBA０．２５±０．０４Ａβ２５－３５GBC GBD０．５１±０．０５Ａβ２５－３５＋Ｐｉｏ０．１０．３６±０．０７＃ １０．３０±０．０３＃＃ １００．２６±０．０４＃＃G8F GB9 GC3 G37 G33 G81１．珋ｘ±ｓ，ｎ＝３．Ｐ＜０．０１，G64 GB8 GB9 GF9 GBA GB9 G4C G59；＃Ｐ＜０．０５，＃＃Ｐ＜０．０１，G64Ａβ２５－３５GBC GBD GB9 G4C G59．G48４ ＷｅｓｔｅｒｎG74 G75 G40 G71 G67ＰｉｏG25Ａβ２５－３５G7F G80 G83 G84 G63 G64 G32G33 G34ｐＪＮＫ１G29 G2A G86 G87 G81 G82．G85 G31１：GB8 GB9 GF9 GBA GB9；G85 G31２：GBC GBDGB9；G85 G31３，４，５：G8F GBE G90Ａβ２５－３５＋Ｐｉｏ０．１，１G58１０μｍｏｌ·Ｌ－１GB9．Ｆｉｇ．４ ＥｆｆｅｃｔｏｆＰｉｏｏｎＡβ２５－３５ｉｎｄｕｃｅｄｐＪＮＫ１ｐｒｏｔｅｉｎｌｅｖｅｌｉｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｂｙＷｅｓｔｅｒｎｂｌｏｔｔｉｎｇ．·７３·GC4 GC5 GC6 GC7 G5E GAA GC8 GC7 G5E GC9 GCA２０１１GCB２GCC GCD２５GCE GCD１GCF ＣｈｉｎＪＰｈａｒｍａｃｏｌＴｏｘｉｃｏｌ，Ｖｏｌ２５，Ｎｏ１，Ｆｅｂ２０１１３ G8F G43G36 G39 GB5 G6F GB6 G81 G70，ＰｉｏGF8 G70 G51 GF9 GDBＡβ２５－３５GFC GEAG53 G47 G48 G65 G2F G30，ＰｉｏGF8 GA8 G43 G54 G5A G4D G53 GF9 GDBＡβ２５－３５GFCGEA G53 G5D G5E GF2 G66 G53 G50 G51，G23 G44 GEC GED G64 GD0 GF2ＰＰＡＲγGCE G92G66 G67，GEF G90 GE8 GEDＰＰＡＲγGCE G92 G54 G50 G4D G53 G51 G52 GD2ＧＷ９６６２GF8 G56 G8F GF9 GDBＰｉｏGF9Ａβ２５－３５GFC GEA G53 G47 G48 G65 GD3GF2 GD4 G87 G50 G53 GEE G75 GEC GED。GB3 G43 G67 G53 G39 GB5 G6F GB6 G81 G70ＰｉｏGF8 G70 G51 GEE G75Ａβ２５－３５GFC GEA G53 G6F GB8 G72ＪＮＫG81 G4E GE0 G92，G94G3F G68 GF8 G70 G51 GEE G75ＪＮＫG8F GF1 GB7 G69 G53 G3E GA2 GF2 G72 GF1ＭＫＫ４G53 G81 G4E。GEF G6A G6B GCBＰｉｏGF9 G47 G48 G65 G53 G24 G25 GEC GED GBD GF8 G64GEE G75ＭＫＫ４／ＪＮＫG6C G41 G6E GBE G29 G2A G66 G67。ＭＫＫ４G42ＪＮＫG8F GF1 GB7 G69 G53 G3E GA2 GF2 G72 GF1［１２］。G7B G66 G39 GB5 G81 G70ＪＮＫG29G2A G6D G64 GCD G6E GB3ＡＤGFC G73 G53 G5D G5E GC1 GC2。ＪＮＫGD0 GF2 G96 GBDG29 G93２G85 G6F G70 GF5 GE1 G6E GC1 GC2 GEC GED：①GB7 G9F G6E GC1 GC2 G7C G67G85 G86 G81 G4E，ＪＮＫG29 G93 G6F GB8 G72ｃＪｕｎG6E GB3 G6E G71 GEF GF1 G72G7D GB2ＡＰ１G53 GF2 G4D GCD GB3 G43 G67 G6E GB3 G59 G44 G6E GC1 GC2 G85 G86 G53G81 G4E，G73ｐ５３、Ｂａｘ、ＦａｓＬGBB［１３］；②G6D G64 G74 GC7 G92 G75 GBEG53 G5D G5E GC1 GC2 G29 G2A。ＰｉｏGEE G75ＪＮＫGD0 GF2 GBD GF8 G42 GF5 GE1 G47G48 G24 G25 GEC GED G74 G75 G5F G43。GF6 GF7 G91 G76 G53 G39 GB5 G73 G62ＰｉｏG29G93 GEE G75ＪＮＫG6C G41 G6E GBE G29 G2A GF9 GDB GD3 G59 GD5 GFC GEA G53 G47 G48G65 GC1 GC2［１４］。G56 G92 G7D G7E G39 GB5 G6F GB6 G68 G81 G70ＰｉｏGF8 GEE G75Ａβ１－４２GFC GEA G53ＭＫＫ４／ＪＮＫG6C G41 G6E GBE G29 G2A G53 GB7 G9F［１１］。G7D G7E G39 GB5 G81 G70，Ｂｃｌ２G77 G7A GEC GED GC8 G5D G5E G79 GD9ｃG53 GB7 G69 G51 G99 G4F G36 GF9，G9B G77Ｂｃｌ２G78 GF8 GEC GED G4F G87 G69 G29G93 GEE G75 GC1 GC2 GEF GF1Ａｐａｆ１，G51 G99 G36 GF9 G53 G5D G5E G79 GD9ｃGF2G72 G79 G5E G85 G86 G95，GB0 G9B GEE G75 GC1 GC2［１５］。G36 G39 GB5 G73 G62ＰｉｏGF8 GF9 GDBＡβ２５－３５GFC GEA G53 G47 G48 G65Ｂｃｌ２G81 G4E G53 G50 G51，G23GBD GF8 G42ＰｉｏG53 G47 G48 G24 G25 GEC GED G53 G7A G43 G74 G75。G7B G69 G7C G39 GB5 G81 G70，ＮＳＡＩＤｓGCD GDB GE5 G36 G29 G93 GD0 GF2ＰＰＡＲγG4BＡβG53 GF5 GE1 GF3 GF4。G56 G59 G73 G4D GDE G72 G58 G7D G21 G7EG41 G53 GD1 GB2 GBC GBD G7D G7E G45 G4B GEDＰＰＡＲγGD0 GD1 GD2 G68 G7B G7F G70 G51GB3 GB4 GCF GB6［１６］。GEF G6A，ＰＰＡＲγG56 G92 G80ＡＤG41 G81 G53 G82 G4DG83 G8A G93 G81 G45 GBD GF8 G42 G43 GB6 G67 G84 G4D G9F G8B GD2，ＰＰＡＲγGD0 GD1GD2 G53 G85 G86 G53 GDB GE5 GEC GED G56 GE5 GE6 G6D G64 G53 G45 G46 G47 G48 G49 G4A G4EG41 G53 GB3 GB4 G45 GBD GF8 G73 G87 GEB G8D GEC GED。ＰｉｏG42ＰＰＡＲγGCE G92G53 GEC G88 G7D G66 G89 G92，G29 G93 GD0 GF2ＰＰＡＲγGCE G92 G9B G73 G87 G8C G8DGEC GED。G36 G39 GB5ＭＴＴG6F GB6 G51 G4D，ＰｉｏG42 G29 G93 GD0 GF2ＰＰＡＲγGCE G92 G56 G8F GEE G75Ａβ２５－３５GFC GEA G92 G2C G2D G2E G47 G48 G65 G5DG5E GF2 G66 G50 G51，G9BＰｉｏG53 G47 G48 G24 G25 GEC GED G42 G31 G8A G8B G64 GD0 GD1ＰＰＡＲγGCE G92 G53 G67 G49，GC1 G56 G96 G76 G7D G7E G45 GB3 G43 G67 G39 GB5。G90 G91 G4C G4D：［１］ ＪｅｌｌｉｎｇｅｒＫＡ．Ｃｌｉｎｉｃｏｐａｔｈｏｌｏｇｉｃａｌａｎａｌｙｓｉｓｏｆｄｅｍｅｎｔｉａｄｉｓｏｒｄｅｒｓｉｎｔｈｅｅｌｄｅｒｌｙ－ａｎｕｐｄａｔｅ［Ｊ］．ＪＡｌｚｈｅｉｍｅｒｓＤｉｓ，２００６，９（３Ｓｕｐｐｌ）：６１７０．［２］ ＴａｎＪ，ＭａｏＧ，ＣｕｉＭＺ，ＫａｎｇＳＣ，ＬａｍｂＢ，ＷｏｎｇＢＳ，ｅｔａｌ．ＥｆｆｅｃｔｓｏｆｇａｍｍａｓｅｃｒｅｔａｓｅｃｌｅａｖａｇｅｒｅｇｉｏｎｍｕｔａｔｉｏｎｓｏｎＡＰＰｐｒｏｃｅｓｓｉｎｇａｎｄＡｂｅｔａｆｏｒｍａｔｉｏｎ：ｉｎｔｅｒｐｒｅｔａｔｉｏｎｗｉｔｈｓｅｑｕｅｎｔｉａｌｃｌｅａｖａｇｅａｎｄａｌｐｈａｈｅｌｉｃａｌｍｏｄｅｌ［Ｊ］．ＪＮｅｕｒｏｃｈｅｍ，２００８，１０７（３）：７２２７３３．［３］ ＫｕｍａｒＳｉｎｇｈＳ，ＰｉｒｉｃｉＤ，ＭｃＧｏｗａｎＥ，ＳｅｒｎｅｅｌｓＳ，ＣｅｕｔｅｒｉｃｋＣ，ＨａｒｄｙＪ，ｅｔａｌ．ＤｅｎｓｅｃｏｒｅｐｌａｑｕｅｓｉｎＴｇ２５７６ａｎｄＰＳＡＰＰｍｏｕｓｅｍｏｄｅｌｓｏｆＡｌｚｈｅｉｍｅｒ′ｓｄｉｓｅａｓｅａｒｅｃｅｎｔｅｒｅｄｏｎｖｅｓｓｅｌｗａｌｌｓ［Ｊ］．ＡｍＪＰａｔｈｏｌ，２００５，１６７（２）：５２７５４３．［４］ ＣａｍａｃｈｏＩＥ，ＳｅｒｎｅｅｌｓＬ，ＳｐｉｔｔａｅｌｓＫ，ＭｅｒｃｈｉｅｒｓＰ，ＤｏｍｉｎｇｕｅｚＤ，ＤｅＳｔｒｏｏｐｅｒＢ．Ｐｅｒｏｘｉｓｏｍｅｐｒｏｌｉｆｅｒａｔｏｒａｃｔｉｖａｔｅｄｒｅｃｅｐｔｏｒｇａｍｍａｉｎｄｕｃｅｓａｃｌｅａｒａｎｃｅｍｅｃｈａｎｉｓｍｆｏｒｔｈｅａｍｙｌｏｉｄｂｅｔａｐｅｐｔｉｄｅ［Ｊ］．ＪＮｅｕｒｏｓｃｉ，２００４，２４（４８）：１０９０８１０９１７．［５］ ｄ′ＡｂｒａｍｏＣ，ＭａｓｓｏｎｅＳ，ＺｉｎｇｇＪＭ，ＰｉｚｚｕｔｉＡ，ＭａｒａｍｂａｕｄＰ，ＤａｌｌａＰｉｃｃｏｌａＢ，ｅｔａｌ．Ｒｏｌｅｏｆｐｅｒｏｘｉｓｏｍｅｐｒｏｌｉｆｅｒａｔｏｒａｃｔｉｖａｔｅｄｒｅｃｅｐｔｏｒｇａｍｍａｉｎａｍｙｌｏｉｄｐｒｅｃｕｒｓｏｒｐｒｏｔｅｉｎｐｒｏｃｅｓｓｉｎｇａｎｄａｍｙｌｏｉｄｂｅｔａｍｅｄｉａｔｅｄｃｅｌｌｄｅａｔｈ［Ｊ］．ＢｉｏｃｈｅｍＪ，２００５，３９１（Ｐｔ３）：６９３６９８．［６］ ＳａｓｔｒｅＭ，ＤｅｗａｃｈｔｅｒＩ，ＲｏｓｓｎｅｒＳ，ＢｏｇｄａｎｏｖｉｃＮ，ＲｏｓｅｎＥ，ＢｏｒｇｈｇｒａｅｆＰ，ｅｔａｌ．Ｎｏｎｓｔｅｒｏｉｄａｌａｎｔｉｉｎｆｌａｍｍａｔｏｒｙｄｒｕｇｓｒｅｐｒｅｓｓｂｅｔａ－ｓｅｃｒｅｔａｓｅｇｅｎｅｐｒｏｍｏｔｅｒａｃｔｉｖｉｔｙｂｙｔｈｅａｃｔｉｖａｔｉｏｎｏｆＰＰＡＲｇａｍｍａ［Ｊ］．ＰｒｏｃＮａｔｌＡｃａｄＳｃｉＵＳＡ，２００６，１０３（２）：４４３４４８．［７］ ＳａｓｔｒｅＭ，ＤｅｗａｃｈｔｅｒＩ，ＬａｎｄｒｅｔｈＧＥ，ＷｉｌｌｓｏｎＴＭ，ＫｌｏｃｋｇｅｔｈｅｒＴ，ｖａｎＬｅｕｖｅｎＦ，ｅｔａｌ．Ｎｏｎｓｔｅｒｏｉｄａｌａｎｔｉｉｎｆｌａｍｍａｔｏｒｙｄｒｕｇｓａｎｄｐｅｒｏｘｉｓｏｍｅｐｒｏｌｉｆｅｒａｔｏｒａｃｔｉｖａｔｅｄｒｅｃｅｐｔｏｒｇａｍｍａａｇｏｎｉｓｔｓｍｏｄｕｌａｔｅｉｍｍｕｎｏｓｔｉｍｕｌａｔｅｄｐｒｏｃｅｓｓｉｎｇｏｆａｍｙｌｏｉｄｐｒｅｃｕｒｓｏｒｐｒｏｔｅｉｎｔｈｒｏｕｇｈｒｅｇｕｌａｔｉｏｎｏｆｂｅｔａｓｅｃｒｅｔａｓｅ［Ｊ］．ＪＮｅｕｒｏｓｃｉ，２００３，２３（３０）：９７９６９８０４．［８］ G2C G8C G8D，G21 G22，G8E G45 G8F，G90 G91，G2E G2F G30，G92 G30 G93，GBB．GC3 GC4 GC5 GC6 GF9 G82 G83 G84βG85 G86 GBB GBC１－４２GFC GEA G53 G59G37 G34 GFD GFE GFF G21 G22 GE4 G2A G57 GE5 GE6 GE7 GE8 G53 G45 G58［Ｊ］．G45 G94 G36G37 G34 G64 G82 G37 G34 G41 G30，２００８，２２（５）：３４８３５４．［９］ G90 G91，G21 G22，G2C G8C G8D，G8E G45 G8F，G2E G2F G30，G92 G30 G93，GBB．GC3 GC4 GC5 GC6 GF9 G82 G83 G84βG85 G86 GBB GBC１－４２GFC GEA G53 G59G37 G2A G57 G47 G48 G5D G5E GC1 GC2 G7C G67 G85 G86 G81 G4E G53 G45 G58［Ｊ］．G45 G94G36 G37 G34 G64 G82 G37 G34 G41 G30，２００８，２２（５）：３５５３６１．［１０］ G8E G45 G8F，G21 G22，G2C G8C G8D，G90 G91，G2E G2F G30，G92 G30 G93，GBB．GC3 GC4 GC5 GC6 GF9 G82 G83 G84βG85 G86 GBB GBC１－４２GFC GEA G53 G59G37 G2A G57 G66 G26 G8F G27 G28 GD0 GF2 G85 G86 GD0 G95ｐ３８G6C G41 G95 GBE G29 G2AG60 G72 G53 G45 G58［Ｊ］．G45 G94 G36 G37 G34 G64 G82 G37 G34 G41 G30，２００８，２２（５）：３６２３６８．［１１］ G2E G2F G30，G96 G97，G21 G22，G26 G2B，G29 G2A G25．GC3 GC4 GC5GC6 GF9 G82 G83 G84βG85 G86 GBB GBC１－４２GFC GEA G53 G59 G37 G2A G57ｃＪｕｎGB7 GAA G2B GD0 G95 G6C G41 G95 GBE G29 G2A G5F G60 G53 GEC GED［Ｊ］．G45 G94 G36 G37G34 G64 G82 G37 G34 G41 G30，２０１０，２４（３）：１７４１７９．［１２］ ＺｏｕＨ，ＬｉＱ，ＬｉｎＳＣ，ＷｕＺ，ＨａｎＪ，ＹｅＺ．ＤｉｆｆｅｒｅｎｔｉａｌｒｅｑｕｉｒｅｍｅｎｔｏｆＭＫＫ４ａｎｄＭＫＫ７ｉｎＪＮＫａｃｔｉｖａｔｉｏｎｂｙｄｉｓｔｉｎｃｔｓｃａｆｆｏｌｄｐｒｏｔｅｉｎｓ［Ｊ］．ＦＥＢＳＬｅｔｔ，２００７，５８１（２）：１９６２０２．·８３·GC4 GC5 GC6 GC7 G5E GAA GC8 GC7 G5E GC9 GCA２０１１GCB２GCC GCD２５GCE GCD１GCF ＣｈｉｎＪＰｈａｒｍａｃｏｌＴｏｘｉｃｏｌ，Ｖｏｌ２５，Ｎｏ１，Ｆｅｂ２０１１［１３］ ＬｉｕＸＭ，ＰｅｉＤＳ，ＧｕａｎＱＨ，ＳｕｎＹＦ，ＷａｎｇＸＴ，ＺｈａｎｇＱＸ，ｅｔａｌ．ＮｅｕｒｏｐｒｏｔｅｃｔｉｏｎｏｆＴａｔＧｌｕＲ６９ｃａｇａｉｎｓｔｎｅｕｒｏｎａｌｄｅａｔｈｉｎｄｕｃｅｄｂｙｋａｉｎａｔｅｉｎｒａｔｈｉｐｐｏｃａｍｐｕｓｖｉａｎｕｃｌｅａｒａｎｄｎｏｎｎｕｃｌｅａｒｐａｔｈｗａｙｓ［Ｊ］．ＪＢｉｏｌＣｈｅｍ，２００６，２８１（２５）：１７４３２１７４４５．［１４］ G29 G2A G25，G21 G22，G98 G62 G99，G23 G24 G25，G2C G2D．GC3 GC4 GC5GC6 GF9 GD3 G59 GD5 GFC GEA G59 G37 G59 G5A G5B G5C G47 G48 G65 G2F G30 G53 GEE G75 GECGED［Ｊ］．G45 G94 G36 G37 G34 G64 G82 G37 G34 G41 G30，２００９，２３（６）：４２３４３０．［１５］ ＰａｎＪ，ＷａｎｇＧ，ＹａｎｇＨＱ，ＨｏｎｇＺ，ＸｉａｏＱ，ＲｅｎＲＪ，ｅｔａｌ．Ｋ２５２ａｐｒｅｖｅｎｔｓｎｉｇｒａｌｄｏｐａｍｉｎｅｒｇｉｃｃｅｌｌｄｅａｔｈｉｎｄｕｃｅｄｂｙ６ｈｙｄｒｏｘｙｄｏｐａｍｉｎｅｔｈｒｏｕｇｈｉｎｈｉｂｉｔｉｏｎｏｆｂｏｔｈｍｉｘｅｄｌｉｎｅａｇｅｋｉｎａｓｅ３／ｃＪｕｎＮＨ２ｔｅｒｍｉｎａｌｋｉｎａｓｅ３（ＪＮＫ３）ａｎｄａｐｏｐｔｏｓｉｓｉｎｄｕｃｉｎｇｋｉｎａｓｅ１／ＪＮＫ３ｓｉｇｎａｌｉｎｇｐａｔｈｗａｙｓ［Ｊ］．ＭｏｌＰｈａｒｍａｃｏｌ，２００７，７２（６）：１６０７１６１８．［１６］ ＬａｎｄｒｅｔｈＧＥ，ＨｅｎｅｋａＭＴ．ＡｎｔｉｉｎｆｌａｍｍａｔｏｒｙａｃｔｉｏｎｓｏｆｐｅｒｏｘｉｓｏｍｅｐｒｏｌｉｆｅｒａｔｏｒａｃｔｉｖａｔｅｄｒｅｃｅｐｔｏｒｇａｍｍａａｇｏｎｉｓｔｓｉｎＡｌｚｈｅｉｍｅｒ′ｓｄｉｓｅａｓｅ［Ｊ］．ＮｅｕｒｏｂｉｏｌＡｇｉｎｇ，２００１，２２（６）：９３７９４４．Ｐｒｏｔｅｃｔｉｖｅｅｆｆｅｃｔｓｏｆｐｉｏｇｌｉｔａｚｏｎｅｏｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｉｎｊｕｒｙｉｎｄｕｃｅｄｂｙａｍｙｌｏｉｄｂｅｔａｐｒｏｔｅｉｎｆｒａｇｍｅｎｔ２５－３５ＪＩＮＹｉｎｇ，ＺＨＡＮＧＺｈｉｊｕａｎ，ＬＩＵＷａｎｚｈｕ，ＳＵＩＨａｉｊｕａｎ，ＬＩＵＺｈｕｏ，ＷＡＮＧＲｕｉ，ＹＡＮＥｎｚｈｉ（ＤｅｐａｒｔｍｅｎｔｏｆＰｈａｒｍａｃｏｌｏｇｙ，ＬｉａｏｎｉｎｇＭｅｄｉｃａｌＵｎｉｖｅｒｓｉｔｙ，Ｊｉｎｚｈｏｕ１２１００１，Ｃｈｉｎａ）Ａｂｓｔｒａｃｔ：ＯＢＪＥＣＴＩＶＥ Ｔｏｉｎｖｅｓｔｉｇａｔｅｗｈｅｔｈｅｒｐｉｏｇｌｉｔａｚｏｎｅ（Ｐｉｏ）ｃａｎｐｒｏｔｅｃｔｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｆｒｏｍａｍｙｌｏｉｄｂｅｔａｐｒｏｔｅｉｎｆｒａｇｍｅｎｔ２５－３５（Ａβ２５－３５）ｉｎｄｕｃｅｄｎｅｕｒｏｔｏｘｉｃｉｔｙ．ＭＥＴＨＯＤＳ ＴｈｅｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｗｅｒｅｉｎｃｕｂａｔｅｄｗｉｔｈＰｉｏ０．０１，０．１，１ａｎｄ１０μｍｏｌ·Ｌ－１ｆｏｒ１ｈ，ｔｈｅｎａｄｄｉｎｇＡβ２５－３５２０μｍｏｌ·Ｌ－１ｆｏｒ２４ｈ．ＧＷ９６６２ｗａｓａｄｄｅｄ３０ｍｉｎｂｅｆｏｒｅＰｉｏ１μｍｏｌ·Ｌ－１ａｄｄｅｄ．ＴｈｅｃｅｌｌｖｉａｂｉｌｉｔｙｗａｓａｓｓｅｓｓｅｄｂｙＭＴＴａｓｓａｙ．ＴｈｅｎｅｕｒｏｎａｌａｐｏｐｔｏｓｉｓｗａｓｑｕａｎｔｉｆｉｅｄｂｙｓｃｏｒｉｎｇｃｅｌｌｓｐｅｒｃｅｎｔａｇｅｗｉｔｈａｐｏｐｔｏｔｉｃｎｕｃｌｅａｒｍｏｒｐｈｏｌｏｇｙａｆｔｅｒＨｏｅｃｈｓｔ３３２５８ｓｔａｉｎｉｎｇ．Ｔｈｅｐｒｏｔｅｉｎｅｘｐｒｅｓｓｉｏｎｓｏｆｐｈｏｓｐｈｏｒｙｌａｔｅｄｍｉｔｏｇｅｎａｃｔｉｖａｔｅｄｐｒｏｔｅｉｎｋｉｎａｓｅｋｉｎａｓｅ４（ＭＫＫ４），ｐｈｏｓｐｈｏｒｙｌａｔｅｄｃＪｕｎＮｔｅｒｍｉｎａｌｋｉｎａｓｅ１（ＰＪＮＫ１），ａｎｄＢｃｌ２ｗｅｒｅｍｅａｓｕｒｅｄｂｙＷｅｓｔｅｒｎｂｌｏｔｔｉｎｇ．ＲＥＳＵＬＴＳ ＴｈｅｄｅｃｒｅａｓｉｎｇｏｆｃｅｌｌｖｉａｂｉｌｉｔｙａｎｄｉｎｃｒｅａｓｅｉｎｇｏｆａｐｏｐｔｏｔｉｃｃｅｌｌｓｉｎｃｕｌｔｕｒｅｄｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｗｅｒｅｏｂｓｅｒｖｅｄｉｎＡβ２５－３５ｇｒｏｕｐｃｏｍｐａｒｅｄｗｉｔｈｎｏｒｍａｌｃｏｎｔｒｏｌｇｒｏｕｐ．Ｔｈｅｃｅｌｌｖｉａｂｉｌｉｔｙｏｆｃｏｒｔｉｃａｌｎｅｕｒｏｎｓｗａｓｄｅｃｒｅａｓｅｄｔｏ（６６．８±１．２）％ｉｎＡβ２５－３５ｍｏｄｅｌｇｒｏｕｐ（Ｐ＜０．０１）．Ｐｉｏ０．１，１ａｎｄ１０μｍｏｌ·Ｌ－１ｃａｎａｎｔａｇｏｎｉｓｔａｇａｉｎｓｔＡβ２５－３５ｉｎｄｕｃｅｄｄｅｃｒｅａｓｉｎｇｏｆｃｅｌｌｖｉａｂｉｌｉｔｙｉｎｃｏｎｃｅｎｔｒａｔｉｏｎｄｅｐｅｎｄｅｎｔｍａｎｎｅｒ，ａｎｄｗｅｒｅ（８１．２±２．９）％，（８７．９±２．２）％ａｎｄ（９８．１±１．９）％，ｒｅｓｐｅｃｔｉｖｅｌｙ．ＧＷ９６６２ｄｉｄｐａｒｔｌｙｉｎｈｉｂｉｔｅｆｆｅｃｔｓｏｆＰｉｏｏｎｃｅｌｌｖｉａｂｉｌｉｔｙ．Ａβ２５－３５ｉｎｄｕｃｅｄｉｎｃｒｅａｓｉｎｇｏｆａｐｏｐｔｏｔｉｃｃｅｌｌｐｅｒｃｅｎｔａｇｅｓｆｒｏｍ（１１．８±１．１）％ｉｎｎｏｒｍａｌｃｏｎｔｒｏｌｇｒｏｕｐｔｏ（６４．６±２．３）％（Ｐ＜０．０１）．Ｐｉｏ０．１，１ａｎｄ１０μｍｏｌ·Ｌ－１ｐｒｅｖｅｎｔｅｄｉｎｃｒｅａｓｅｏｆａｐｏｐｔｏｔｉｃｃｅｌｌｓｐｅｒｃｅｎｔａｇｅ，ｒｅｓｐｅｃｔｉｖｅｌｙ（５８．３±１．２）％，（５２．６±１．３）％ａｎｄ（４１．５±１．５）％．Ｆｕｒｔｈｅｒｍｏｒｅ，ｎｅｕｒｏｎｓｉｎｃｕｂａｔｉｏｎｗｉｔｈＡβ２５－３５ｆｏｒ２４ｈｉｎｄｕｃｅｄｔｈｅｄｅｃｒｅａｓｅｏｆＢｃｌ２ｅｘｐｒｅｓｓｉｏｎ，ｉｎｃｒｅａｓｅｓｉｎｐｈｏｓｐｈｏＭＫＫ４ａｎｄｐＪＮＫ１ｅｘｐｒｅｓｓｉｏｎｓ．ＰｒｅｔｒｅａｔｍｅｎｔｗｉｔｈＰｉｏｓｉｇｎｉｆｉｃａｎｔｌｙｉｎｈｉｂｉｔｅｄｒｅｄｕｃｔｉｏｎｏｆＢｃｌ２ｅｘｐｒｅｓｓｉｏｎｉｎｄｕｃｅｄｂｙＡβ２５－３５．Ｐｉｏ０．１，１ａｎｄ１０μｍｏｌ·Ｌ－１ａｌｓｏｉｎｈｉｂｉｔｅｄＡβ２５－３５ｉｎｄｕｃｅｄｉｎｃｒｅａｓｉｎｇｉｎＭＫＫ４ａｎｄＪＮＫ１ｐｈｏｓｐｈｏｒｙｌａｔｉｏｎ．ＣＯＮＣＬＵＳＩＯＮ ＰｉｏｐｒｏｔｅｃｔｓｃｏｒｔｉｃａｌｎｅｕｒｏｎｓａｇａｉｎｓｔＡβ２５－３５ｉｎｓｕｌｔｔｈｒｏｕｇｈＰＰＡＲγｒｅｃｅｐｔｏｒａｃｔｉｖａｔｉｏｎａｎｄｕｐｒｅｇｕｌａｔｉｎｇＢｃｌ２ｅｘｐｒｅｓｓｉｏｎａｎｄｉｎｈｉｂｉｔｉｎｇＭＫＫ４／ＪＮＫｐａｔｈｗａｙ．Ｋｅｙｗｏｒｄｓ：ｐｉｏｇｌｉｔａｚｏｎｅ；ａｍｙｌｏｉｄｂｅｔａｐｒｏｔｅｉｎｆｒａｇｍｅｎｔ；Ａｌｚｈｅｉｍｅｒｄｉｓｅａｓｅ；ａｐｏｐｔｏｓｉｓ；ｃＪｕｎＮｔｅｒｍｉｎａｌｋｉｎａｓｅｓ Ｆｏｕｎｄａｔｉｏｎｉｔｅｍ：ＴｈｅｐｒｏｊｅｃｔｓｓｕｐｐｏｒｔｅｄｂｙＥｄｕｃａｔｉｏｎＤｅｐａｒｔｍｅｎｔｏｆＬｉａｏｎｉｎｇＰｒｏｖｉｎｃｅ（ＬＴ２０１００６４）；ａｎｄＰｒｏｖｉｎｃｉａｌＮａｔｕｒａｌＳｃｉｅｎｃｅＦｏｕｎｄａｔｉｏｎｏｆＬｉａｏｎｉｎｇＰｒｏｖｉｎｃｅ（２００４２１７１） Ｃｏｒｒｅｓｐｏｎｄｉｎｇａｕｔｈｏｒ：ＪＩＮＹｉｎｇ，Ｅｍａｉｌ：ｊｙｊｉｎｙｉｎｇ＠ｙａｈｏｏ．ｃｏｍ．ｃｎ，Ｔｅｌ：（０４１６）４６７３４０９（G9A G9B G3B G76：２０１００１１５ GA2 GCE G3B G76：２０１００８１８）（G36 G9C G9D G9E：G9F GA0）·９３·GC4 GC5 GC6 GC7 G5E GAA GC8 GC7 G5E GC9 GCA２０１１GCB２GCC GCD２５GCE GCD１GCF ＣｈｉｎＪＰｈａｒｍａｃｏｌＴｏｘｉｃｏｌ，Ｖｏｌ２５，Ｎｏ１，Ｆｅｂ２０１１

本文档只以信息传播为目的，不代表本站认同其观点和立场，以及认同文中所述皆为事实。